Journal: Advanced Science

Article Title: Polyplex Nanomicelle‐Mediated Pgc‐1α4 mRNA Delivery Via Hydrodynamic Limb Vein Injection Enhances Damage Resistance in Duchenne Muscular Dystrophy Mice

doi: 10.1002/advs.202409065

Figure Lengend Snippet: Expression levels of PGC‐1α and associated genes in dystrophic muscles. A) Western blotting of PGC‐1α1 and PGC‐1α4 protein levels ( n = 3 mice). B) Quantitative analysis of the western blot bands normalized to GAPDH. C) Relative mRNA expression levels of total Pgc‐1α (Pgc‐1α1,2,3, and 4), Pgc‐1α1, and Pgc‐1α4. D–K) Relative mRNA levels of genes associated with PGC‐1α in various aspects including muscle hypertrophy (D), myogenic induction (E), mitochondrial activity (F), angiogenesis (G), neuromuscular junction (NMJ) (H), myofiber type (I), metabolism (J), and inflammation (K). All analyses were performed using plantar flexor muscles. Data are presented as mean ± SEM ( n = 6 mice), and Welch's t ‐test was used ( *** p < 0.001, ** p < 0.01, and * p < 0.05).

Article Snippet: Membranes were blocked with 5% non‐fat dry milk and incubated with primary antibodies against PGC‐1α (sc‐518025, Santa Cruz; ST1202, Calbiochem) and loading controls GAPDH (D16H11, Cell Signaling Technology) and α‐tubulin (T6199, Sigma–Aldrich).

Techniques: Expressing, Muscles, Western Blot, Activity Assay

Journal: Advanced Science

Article Title: Polyplex Nanomicelle‐Mediated Pgc‐1α4 mRNA Delivery Via Hydrodynamic Limb Vein Injection Enhances Damage Resistance in Duchenne Muscular Dystrophy Mice

doi: 10.1002/advs.202409065

Figure Lengend Snippet: General upregulation of dysregulated genes after nanomicelle‐delivered Pgc‐1α4 mRNA treatment. A,B) Western blotting images of PGC‐1α4 protein expression on day 2 (A) and day 7 (B). C) Quantification of western blotting bands normalized to GAPDH (for day 2, n = 5 for the LNP + α4 group, n = 6 for all other groups; for day 7, n = 4 for the LNP + α4 group, n = 5 for the micelle + Fluc group, and n = 6 for the micelle + α4 group). D–L) Relative mRNA levels of genes associated with PGC‐1α on day 2 and day 7. All analyses were performed using plantar flexor muscle samples. Data are presented as mean ± SEM ( n = 6 mice), and one‐way ANOVA followed by Tukey's post hoc test was used ( *** p < 0.001, ** p < 0.01, and * p < 0.05).

Article Snippet: Membranes were blocked with 5% non‐fat dry milk and incubated with primary antibodies against PGC‐1α (sc‐518025, Santa Cruz; ST1202, Calbiochem) and loading controls GAPDH (D16H11, Cell Signaling Technology) and α‐tubulin (T6199, Sigma–Aldrich).

Techniques: Western Blot, Expressing